Carolin A. Müller

Single-molecule landscape of DNA replication pausing

The paper identifies replication pause sites in a eukaryotic genome using long-read nanopore sequencing and finds they are enriched near transcribed regions and correlate with transcription and R-loop levels.

Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.

Resistance to miltefosine results from amplification of the RTA3 floppase or inactivation of flippases in Candida parapsilosis

This study identifies that resistance to miltefosine in Candida parapsilosis arises from RTA3 gene amplification or flippase inactivation, altering plasma membrane lipid asymmetry.

Sir2 mitigates an intrinsic imbalance in origin licensing efficiency between early and late replicating euchromatin

The paper shows Sir2 regulates MCM complex distribution in yeast chromosomes to ensure proper origin licensing and genome duplication.

Inter-species conservation of organisation and function between non-homologous regional centromeres

The paper shows that centromere DNA from non-homologous fission yeast species can be recognized and function across species, despite lacking sequence conservation.

Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads

This paper introduces a method to measure DNA replication dynamics on single molecules using nanopore sequencing and applies it to yeast to discover new replication origins.