An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR mediated homologous recombination
Oguz Kanca, Jonathan D. Zirin, Yanhui Hu…·Hugo Jozef Bellen
The paper develops new CRISPR-based constructs for Drosophila gene tagging, enabling efficient gene modification with a 70-80% success rate regardless of exon-intron structure.