This study elucidates the molecular mechanism of IF1 and IF2 in bacterial translation initiation using cryo-EM and fast-kinetics, showing their roles in subunit association, GTP hydrolysis, and Pi release.

bioRxiv · Jun 29, 2026 · PreprintBiochemistry

Mechanistic insights into E. coli recovery from growth arrest

Ahmed H. Hassan, Yuko Nakano, Howard Gamper, Isao Masuda, Ludmila Veselá, Matyáš Pinkas, Sathya Narayanan Nagarajan, Jonathan E. Dworkin, Gregor M. Blaha, Ya‐Ming Hou, Gabriel Demo

This study investigates how E. coli recovers from growth arrest by analyzing the role of RimM in ribosome maturation and recovery mechanisms.

Nature Communications · Apr 11, 2026Peer-reviewedMolecular Biology

Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB

Karina Jouravleva, Dmitrij Golovenko, Gabriel Demo, Robert C. Dutcher, Traci M. Tanaka Hall, Phillip D. Zamore, Andrei A. Korostelev

The paper reports cryo-EM structures of Drosophila Dicer-1 and Loqs-PB complex during pre-miRNA processing, revealing the mechanism of miRNA biogenesis.

Molecular Cell · Sep 30, 2022Peer-reviewedMolecular Biology

Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP

Christine E. Carbone, A.B. Loveland, Howard Gamper, Ya‐Ming Hou, Gabriel Demo, Andrei A. Korostelev

The paper uses time-resolved cryo-EM to visualize GTP-catalyzed ribosomal translocation by EF-G, revealing intermediate structures and mechanisms of tRNA and mRNA movement.

Nature Communications · Dec 13, 2021Peer-reviewedMolecular Biology

Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation

Gabriel Demo, Howard Gamper, A.B. Loveland, Isao Masuda, Christine E. Carbone, Egor Svidritskiy, Ya‐Ming Hou, Andrei A. Korostelev

The paper determines the structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation using cryo-EM structures.

Nature Communications · Jul 30, 2021Peer-reviewedMolecular Biology