Yves Jacob

The paper maps binary SARS-CoV-2 protein interactions with host proteins, identifying key processes like immune signaling and ubiquitination, and suggests therapeutic targets.

The paper presents a framework combining two high-throughput interaction assays to distinguish direct from indirect protein interactions in complexes.

The paper constructs a high-quality yeast binary interactome map and compares inner- and outer-complexome organization, finding the outer-complexome contains more interactions and exhibits higher functional heterogeneity.

Proximity-dependent biotinylation of 27 SARS-CoV-2 proteins in A549 cells maps 7810 virus-host proximity interactions across 2242 host proteins, including membrane-associated partners missed by affinity purification.

Proximity-dependent biotinylation (BioID) of 28 SARS-CoV-2 proteins in human cells maps 5,415 proximal interactions with 2,128 host targets across basal and poly(I:C) conditions.

The paper introduces an assayome concept to optimize binary interactome mapping by combining multiple assay versions, achieving high true positive detection with minimal assays.

The paper presents HuRI, a comprehensive human protein-protein interaction map, and demonstrates its utility in understanding cellular function and disease mechanisms.