Charlotte R. Pfeifer

Live cell monitoring for factors affecting genome variation

The paper monitors live cell fluorescence to quantify aneuploidy-inducing perturbations in lung cancer and iPS cells, revealing increased chromosome mis-segregation and rare fluorescent-null cells.

Cell cycle repression and DNA repair defects follow constricted migration

The paper shows that constricted migration causes nuclear rupture and DNA damage, which are linked to cell cycle repression and DNA repair defects in cancer cells.

DNA damage in 3D constricted migration or after lamin-A depletion in 2D: shared mechanisms of repair factor mis-localization under nuclear stress

Constricted cell migration causes nuclear lamina damage, DNA breaks, and squeeze-out of repair factors

The paper shows that constricted cell migration in stiff tissues causes nuclear lamina damage, DNA breaks, and loss of repair factors, leading to genomic instability.