Sebastian Wenk

Evolution-assisted engineering of formate assimilation via the formyl phosphate route in Escherichia coli

This study establishes the formyl phosphate route for formate assimilation in E. coli through adaptive evolution and rational engineering, enabling formate-dependent growth.

The Serine Shunt enables formate conversion to formaldehyde in vivo

The paper introduces the Serine Shunt as a novel formate reduction pathway in E. coli, demonstrating its in vivo activity and efficiency for converting formate to formaldehyde.

Paving the way for synthetic C1- metabolism in Pseudomonas putida through the reductive glycine pathway

This paper engineers Pseudomonas putida to assimilate C1 compounds like formate, methanol, and CO2 through the reductive glycine pathway, enabling growth on these substrates as sole feedstocks.

Synthetic carbon fixation via the autocatalytic serine threonine cycle

The paper engineers a synthetic C1-assimilation cycle in E. coli to enable growth on formate, demonstrating the feasibility of synthetic carbon fixation from CO2.