Steffen N. Lindner

This paper develops a microbial platform to study Rubisco oxygenase activity in vivo by linking it to bacterial growth, enabling genetic screening and evolution of enzyme variants.

This paper demonstrates the efficient conversion of formate into methyl groups in engineered Escherichia coli using SAM-dependent methyltransferases, achieving over 70% formate-derived methyl groups.

This paper presents a computational workflow for designing and testing E. coli metabolic sensors that detect glyoxylate and glycolate across a wide concentration range, with applications in bioengineering and environmental monitoring.

This study replaces the glycerate pathway with the β-hydroxyaspartate cycle in Pseudomonas putida, resulting in faster growth and higher biomass yield on ethylene glycol.

The paper engineers genome-reduced Pseudomonas putida strains to assimilate formate as a carbon source using a modular approach and adaptive evolution, achieving faster growth and two distinct catabolic pathways.

The paper engineers a synthetic C1-assimilation cycle in E. coli to enable growth on formate, demonstrating the feasibility of synthetic carbon fixation from CO2.

This paper optimizes an E. coli strain for formate-based bioproduction using the reductive glycine pathway, achieving improved growth and lactate production.

The paper studies the flexibility of the amination network in E. coli by identifying key enzymes and amino acids involved in ammonium assimilation and glutamate production.

This study identifies alternative glycolytic pathways in E. coli that bypass canonical EMP glycolysis, showing that the serine shunt is selected for in adaptive evolution.

The paper evolves mutant strains of Corynebacterium glutamicum that utilize fructose more efficiently, leading to increased biomass and L-lysine production.

This study identifies a new carbon fixation pathway in E. coli using only native enzymes and demonstrates its in vivo feasibility through genetic engineering.