Jörg Kahnt

Directed evolution of the Fe-nitrogenase for CO2 reduction to hydrocarbons

This study enhances Fe-nitrogenase's ability to convert CO2 to hydrocarbons through directed evolution, increasing CH4 production and expanding the product spectrum to include ethane and CO.

Interface swapping orchestrates carbon transfer in the archaeal acetyl-CoA decarbonylase/synthase

The paper investigates the structure and dynamics of the archaeal ACDS complex, revealing a mechanism for efficient carbon transfer through transient interactions rather than stable complexes.

Combining evolution and machine learning-guided pathway optimization to engineer a novel methylsuccinate module for synthetic C1 metabolism in vivo

This paper combines adaptive laboratory evolution and machine learning to engineer a methylsuccinate module for synthetic C1 metabolism in E. coli, achieving growth in three weeks versus six months with evolution alone.

Evolution-assisted engineering of formate assimilation via the formyl phosphate route in Escherichia coli

This study establishes the formyl phosphate route for formate assimilation in E. coli through adaptive evolution and rational engineering, enabling formate-dependent growth.

Atomic resolution structures of the methane-activating enzyme in anaerobic methanotrophy reveal extensive post-translational modifications.

The paper determines atomic structures of MCRs from anaerobic methanotrophic archaea, revealing conserved structures and post-translational modifications.

Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with an oxygen-stress responsive small protein

The paper presents a cryo-EM structure of the Mtr complex from Methanosarcina mazei and identifies a small ORF protein, MtrI, involved in oxygen stress response.

System-level characterization of engineered and evolved formatotrophic E. coli strains

This study investigates why engineered E. coli strains for formate utilization do not reach theoretical biomass yields and identifies metabolic bottlenecks and evolutionary changes to improve them.

Construction of multicellular yeast networks using the communication toolkit with variable specificity and attenuation

The paper develops a yeast communication toolkit for constructing multicellular networks with variable specificity and attenuation, demonstrating its application in logic gate-like networks and promiscuity analysis.

Drosophila carboxypeptidase D (SILVER) is a key enzyme in neuropeptide processing required to maintain locomotor activity levels and survival rate

This study identifies dCPD as a critical enzyme for neuropeptide processing in Drosophila, showing its role in behavior and survival.