Vittorio Rainaldi

This paper combines adaptive laboratory evolution and machine learning to engineer a methylsuccinate module for synthetic C1 metabolism in E. coli, achieving growth in three weeks versus six months with evolution alone.

This paper identifies novel carbon fixation pathways using bioretrosynthetic analysis and expert knowledge, finding several that could outperform the Calvin cycle in biomass yield and do not require vitamin B12.

This paper designs and tests synthetic CO2 fixation pathways in E. coli, demonstrating their potential to outperform natural pathways.

The paper identifies a recursive isoleucine biosynthesis pathway in E. coli driven by the promiscuous activity of acetohydroxyacid synthase II, using glyoxylate and pyruvate directly instead of threonine.

The paper engineers a synthetic C1-assimilation cycle in E. coli to enable growth on formate, demonstrating the feasibility of synthetic carbon fixation from CO2.

This paper optimizes an E. coli strain for formate-based bioproduction using the reductive glycine pathway, achieving improved growth and lactate production.