Heewhan Shin

Structural basis of directionality control in large serine integrases

The paper reports structural insights into how large serine integrases control directionality during DNA integration and excision using recombination directionality factors.

Continuous negative autoregulation fine-tunes dosage-sensitive transcription factor expression to maintain post-mitotic neuron identity

This study shows that negative autoregulation of the TF UNC-3 maintains precise expression levels in post-mitotic neurons, counterbalanced by positive input from CEH-20/PBX, ensuring cell identity.

Functional relevance of CASP16 nucleic acid predictions as evaluated by structure providers

Structure providers for 18 CASP16 nucleic acid targets find blind predictions capture secondary structure but miss non-canonical interactions, backbone kinks, ligand sites, and intermolecular interfaces.

Identification of cognate recombination directionality factors for large serine recombinases by virtual pulldown

The paper identifies cognate recombination directionality factors (RDFs) for large serine recombinases using AlphaFold2-multimer and experimental validation.

Large serine integrases utilise scavenged phage proteins as directionality cofactors

The paper identifies that RDFs for TG1 and Bxb1 integrases function as scavenged phage proteins, with TG1 RDF acting as a functional SSB protein.

Variable orthogonality of RDF-large serine integrase interactions within the phiC31 family

The paper evaluates the orthogonality of integrase-RDF pairs in phiC31 family enzymes, finding varying selectivity and directionality in their recombination reactions.