Phoebe A. Rice

Structural basis of directionality control in large serine integrases

The paper reports structural insights into how large serine integrases control directionality during DNA integration and excision using recombination directionality factors.

Antagonism by the Type VI secretion system of Bacteroides fragilis is controlled by a TetR family regulator and released small molecule

This paper identifies a small molecule regulated by a TetR family regulator that controls the GA3 T6SS and immunity genes in Bacteroides fragilis, enabling coordinated antagonism and defense.

Functional relevance of CASP16 nucleic acid predictions as evaluated by structure providers

Structure providers for 18 CASP16 nucleic acid targets find blind predictions capture secondary structure but miss non-canonical interactions, backbone kinks, ligand sites, and intermolecular interfaces.

Identification of cognate recombination directionality factors for large serine recombinases by virtual pulldown

The paper identifies cognate recombination directionality factors (RDFs) for large serine recombinases using AlphaFold2-multimer and experimental validation.

Large serine integrases utilise scavenged phage proteins as directionality cofactors

The paper identifies that RDFs for TG1 and Bxb1 integrases function as scavenged phage proteins, with TG1 RDF acting as a functional SSB protein.

Variable orthogonality of RDF-large serine integrase interactions within the phiC31 family

The paper evaluates the orthogonality of integrase-RDF pairs in phiC31 family enzymes, finding varying selectivity and directionality in their recombination reactions.