Lindsey Doyle

The paper analyzes homing endonuclease genes, identifies their activity levels, and reconstructs ancestral forms showing improved function and structural insights.

This study investigates the structural and functional properties of BrxC, a conserved AAA+ ATPase in the BREX phage restriction system, revealing its role in mediating competing protein associations and DNA binding that regulate host methylation and phage restriction.

The paper shows that restricted phage infection primes bacterial immunity to defeat counterdefenses by increasing defense protein abundance and altering host-phage stoichiometry.

This paper designs sequence-specific DNA-binding proteins computationally and validates them experimentally, achieving high specificity and affinity for target sequences.

This study identifies and characterizes the PglZ:BrxB sub-complex of BREX phage defence systems, revealing PglZ as a metal-dependent nuclease that contributes to phage defence.

The paper identifies and characterizes the BrxR protein and its gene in a BREX phage restriction system from Acinetobacter, showing its role in restriction and cis regulation.