Vasco M. Barreto

Endogenous intronic RNA tightly controls Cas9/CRISPR-mediated gene editing in human cells

The paper introduces intcgRNA, a conditional guide RNA that uses endogenous intronic RNA to control CRISPR/Cas9 editing, showing improved specificity in human cells.

Single-cell reconstitution reveals persistence of clonal heterogeneity in the murine hematopoietic system

The paper investigates the persistence of allelic expression patterns in murine hematopoietic stem cells and their differentiated B-cell clones, finding that most allele-specific transcriptional states are established de novo during differentiation.

Partitioning stable and unstable expression level variation in cell populations: a theoretical framework with application to the T cell receptor

The paper develops a framework to distinguish stable and unstable expression variation in cell populations and applies it to TCR expression in CD4 T cells, finding 70% stable variance in polyclonal populations and over 80% volatile variance in isogenic populations.

A CyclinB2-Cas9 fusion promotes the homology-directed repair of double-strand breaks

This paper describes a CyclinB2-Cas9 fusion protein that enhances homology-directed repair of double-strand breaks by promoting Cas9 activity during the G2 phase of the cell cycle.