Evangelos D. Karousis

The paper presents a scalable method for preparing cell-type-specific lysates from human cell lines to improve cell-free translation systems.

This study investigates how SARS-CoV-2 and MERS-CoV Nsp1 proteins affect host mRNA degradation, revealing that Nsp1 binding to ribosomes triggers degradation independently of translation inhibition.

This paper introduces a dual centrifugation method to produce human translation-competent lysates, enabling efficient mRNA translation in both cap-dependent and IRES-mediated ways.

The paper uses long-read sequencing to identify novel NMD-sensitive mRNAs and reveals features of their biogenesis and biological role.

This study shows SARS-CoV-2 Nsp1 binds to the 40S ribosomal subunit to inhibit translation by blocking mRNA entry, while viral mRNA enhances its own translation.

This study challenges the prevailing model of NMD by showing that ribosome stalling at stop codons does not occur during NMD activation in vitro or in vivo.