Phillip Kyriakakis

OpenEvo is an open-source platform for automated evolution experiments that enables directed evolution with cost-effective, user-friendly hardware and software.

This paper demonstrates that a DUX4 DNA-binding domain fused to a human KRAB domain can effectively repress DUX4-FL transcriptional activity in vitro, and develops computational models to explore its therapeutic potential for FSHD.

This study identifies glucose 6-phosphate (G6P) as a second trigger for Glk1p polymerization and shows that ADP causes filament disassembly, linking metabolic state to enzyme polymerization.

This paper introduces a method to assemble synthetic T7 phage genomes in vitro without restriction enzymes, enabling modular DNA insertion.

This paper introduces a light-guided Rabies Virus tracing system for neural circuits, enabling controlled, nontoxic, and scalable transsynaptic tracing.