Bi‐Chang Chen

Physicochemical balancing facilitates fast cyclic immunolabeling in centimeter-scale FFPE tissues

The paper introduces FIDELITY, a method for rapid cyclic immunolabeling of FFPE tissues, enabling multi-cycle protein profiling in intact specimens.

Dodecagon light-sheet fluorescence microscopy for large-volume imaging without striping artifacts

This paper introduces dodecagon light-sheet fluorescence microscopy (dodecaLSFM) to eliminate striping artifacts in large-volume imaging, demonstrating its effectiveness in imaging whole mouse brain vasculature and combining with expansion microscopy for 3D cellular resolution.

High-resolution volumetric intravital imaging reveals asymmetric serotonin-dependent post-shock activity in the Drosophila brain

This paper introduces vSPIM, a high-speed intravital imaging technique, and uses it to discover asymmetric serotonin-dependent post-shock activity in Drosophila brains.

Shared Alteration of Whole-Brain Connectivity and Olfactory Deficits in Multiple Autism Mouse Models

The paper identifies shared connectivity deficits and olfactory impairments across multiple autism mouse models using whole-brain mapping and AI analysis.

Ki-67 and CDK1 Control the Dynamic Association of Nuclear Lipids with Mitotic Chromosomes

This study shows Ki-67 and CDK1 regulate nuclear lipid distribution around chromosomes during mitosis, ensuring proper chromosome segregation and genome integrity.

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

This paper introduces HIF-Clear, a method for clearing and labeling FFPE brain specimens, enabling analysis of neuronal circuitry in whole brains.

Macrophages break interneuromast cell quiescence by intervening the inhibition of Schwann cells in zebrafish lateral line

The paper shows macrophages help regenerate neuromasts in zebrafish by disrupting Schwann cell inhibition of interneuromast cells during injury.

Rule-governed Dynamic Stochastic Equilibration of Multicellular Motion In Vivo During Olfactory Neurogenesis

The paper tracks progenitor cell movements in zebrafish embryos to uncover coordination mechanisms between neural crest and placode-derived lineages during olfactory neurogenesis.

Visualization of axonal protein allocation in Drosophila with whole brain localization microscopy

The paper uses super-resolution imaging to show that learning-induced proteins are localized to specific axonal sectors in Drosophila mushroom bodies, linking neural activity patterns to long-term memory formation.