Akandé Rouchidane Eyitayo

This paper describes methods to produce membrane-embedded Bcl-2 family proteins using cell-free synthesis in lipid bilayer nanodiscs for structural and functional studies.

The paper shows lipid droplets delay regulated cell death by sequestering mitochondrial effectors like Bax, thereby reducing apoptosis execution in cancer and germ cells.

This study investigates Bax membrane insertion in nanodiscs, showing Tom22-dependent multi-step processes involving hydrophobic motifs and conformational changes.