This paper introduces IMaP, a method to analyze tau proteoforms at the single-molecule level, revealing ordered phosphorylation patterns and disease-specific modifications.

Nature Methods · Sep 1, 2026Peer-reviewedBiochemistry

WRN Inhibition Leads to its Chromatin-Associated Degradation Via the PIAS4-RNF4-p97/VCP Axis

Fernando Rodríguez-Pérez, Dean E. Natwick, Lauren Schiff, David Trombley McSwiggen, Alec Basil Heckert, Melina Huey, Huntly M. Morrison, Mandy Loo, Rafael G. Miranda, John Filbin, José Ortega, Kayla Van Buren, Danny Murnock, Arnold Tao, Renee Butler, , Stephen E. Basham

This study identifies a novel regulatory mechanism for WRN degradation via the PIAS4-RNF4-p97/VCP axis in MSI-H cancer cells using single molecule tracking and inhibitor screening.

Nature Communications · Jul 18, 2024Peer-reviewedMolecular Biology

Targeted protein degradation systems to enhance Wnt signaling

Parthasarathy Sampathkumar, Heekyung Jung, Hui Chen, Zhengjian Zhang, Nicholas Suen, Yiran Yang, Zhong Xian Huang, Tom Lopez, Robert Benisch, Sung‐Jin Lee, Jay Ye, Wen‐Chen Yeh, Yang Li

The paper describes the development of new antibodies that enhance Wnt signaling through targeted protein degradation by sequestering E3 ligases.

eLife · Feb 8, 2024Peer-reviewedSynthetic Biology