Joshua H. Mendez

This study uses cryo-EM to determine the structure of a light-regulated bacterial two-component system, revealing how light affects signaling through changes in histidine kinase and response regulator interactions.

The paper determines the 2.78 Å resolution cryo-EM structure of Escherichia coli SiR, revealing the minimal interface for diflavin reductase binding and distinguishing between hemoprotein and ferredoxin partner interactions.

This study characterizes IDE conformational dynamics using cryo-EM and MD simulations, identifying key residues and mechanisms that modulate enzyme activity.

This study uses time-resolved cryo-EM to visualize early intermediates in bacterial RNA polymerase promoter melting, revealing key steps in the formation of the transcription-competent open complex.

The paper reports Cryo-EM structures of bacterial phytochromes in different light states, revealing insights into their signaling mechanisms.

This paper introduces Smart Leginon Autoscreen, an automated cryoEM grid screening system that reduces operator time from 6 hours to 10 minutes for 12 grids using machine learning and computer vision.