Edward T. Eng

Cryoprotectants-assisted plunge freezing of thick brain tissue specimens for targeted physiologically relevant cryo-imaging in situ

This paper develops a cryoprotectant-assisted plunge freezing method for preserving thick mouse brain tissue for cryo-imaging, enabling high-resolution visualization of cellular structures and validating their physiological relevance.

Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci

The paper determines structures of the Cas1-2/3 complex to elucidate how it captures, delivers, and integrates foreign DNA into CRISPR loci.

Multi-species cryoEM calibration and workflow verification standard

The paper introduces a multi-species cryoEM calibration sample to validate instrument performance and image processing workflows.

CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization

This paper introduces CryoCycle, a method for blotting, vitrifying, and reusing clipped cryoEM grids to reduce costs and improve access to cryoEM.

Smart Leginon enables cryoEM screening automation across multiple grids

This paper introduces Smart Leginon Autoscreen, an automated cryoEM grid screening system that reduces operator time from 6 hours to 10 minutes for 12 grids using machine learning and computer vision.

The impact of data reduction and lossy image formats on electron microscope images

This study examines how data compression and lossy image formats affect cryoEM reconstruction quality, finding that compressed data can still yield sub-2Å reconstructions.

Benchmarking cryo-EM single particle analysis workflow

The paper benchmarks cryo-EM single particle analysis workflows using a standard sample and procedures to ensure high-resolution map production.

Structural basis of transcription inhibition by fidaxomicin (lipiarmycin A3)

This paper reports the cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme bound to fidaxomicin, revealing its unique binding site and mechanism of transcription inhibition.

Spotiton: New Features and Applications

The paper describes new features and applications of Spotiton, a cryoEM sample vitrification instrument, showing it produces high-quality specimens with no apparent sample damage.