Anil Kumar Challa

An integrated workflow for dual-guide CRISPR-Cas9 targeting and amplicon-based genotyping of Pla2g6 in primary mouse bone marrow-derived macrophages

This paper describes a workflow for dual-guide CRISPR-Cas9 editing of Pla2g6 in mouse BMDMs, showing high editing efficiency and specific deletion patterns.

ER Stress-Induced beta-Cell Apoptosis is Linked to Novel Select Lipid Signaling at the Transcriptional Level: Implications in T1D Development

This study identifies a novel lipid signaling pathway involving iPLA2β that links ER stress to β-cell apoptosis in T1D, revealing transcriptional regulation by iDLs and potential therapeutic targets.

Transcriptional regulation of structural and functional adaptations in a developing adulthood myocardium

The paper investigates transcriptional changes in mouse myocardium during post-natal to adult transition, finding significant gene expression shifts and structural-functional adaptations.

Analysis of novel domain-specific mutations in the zebrafish ndr2/cyclops gene generated using CRISPR-Cas9 RNPs

This study generates domain-specific mutations in the zebrafish ndr2 gene using CRISPR-Cas9 RNPs, resulting in cyclopia and providing insights into the gene's structure-function relationship.