The paper presents the cryo-EM structure of the MecA/ClpC/ClpP complex from Staphylococcus aureus, revealing how MecA facilitates substrate transfer and how ClpC/ClpP interactions are regulated by ATPase activity.

Communications Biology · Oct 14, 2025Peer-reviewedBiochemistry

Unmasking AlphaFold: integration of experiments and predictions in multimeric complexes

Claudio Mirabello, Björn Wallner, Björn Nystedt, Stavros Azinas, Marta Carroni

This study introduces AF_unmasked, a method that integrates experimental data with AlphaFold predictions to improve the accuracy of large protein complex structures.

bioRxiv · Sep 20, 2023 · PreprintBioinformatics

Autophagic signaling promotes systems-wide remodeling in skeletal muscle upon oncometabolic stress

Anja Karlstaedt, Heidi Vitrac, Rebecca Salazar, Benjamin D. Gould, Daniel Soedkamp, Weston R. Spivia, Koen Raedschelders, An Q Dinh, Anna G. Guzman, Lin Tan, Stavros Azinas, David J.R. Taylor, Walter B. Schiffer, Daniel McNavish, Helen B. Burks, , Heinrich Taegtmeyer

This study shows that D2-HG induces cachexia in mice by disrupting NAD+ homeostasis and autophagy, revealing metabolic vulnerabilities in skeletal muscle.

bioRxiv · Oct 13, 2020 · PreprintSystems Biology