Adam T. Smiley

This paper introduces NovoCAST, a new RNA-guided transposon system designed through computational protein design, enabling efficient and programmable DNA integration in human cells.

This study engineers HUH-tags to form sequence-directed covalent bonds with RNA, enhancing their reactivity through directed evolution.

The paper shows that a foreign tandem repeat can induce heterochromatin formation in Drosophila, with temperature-dependent silencing and distinct biochemistry compared to canonical PEV.

This study develops a Notch inhibitory nanobody and demonstrates a strategy to enhance its efficacy through fusion with an Aerolysin domain.

HUHgle is a tool for designing and optimizing HUH-tag mediated protein-ssDNA conjugates, predicting formation efficiency and specificity.

This paper presents a digital imaging workflow for standardized, multiplexed quantitative analysis of adult Drosophila eye phenotypes, reducing variability and enabling precise morphometric comparisons.