Pim P. B. America

The paper investigates how RfaH and NusG regulate RNA polymerase pausing during transcription of long operons in E. coli, showing RfaH extends transcription runs and compensates for NusA's pause-inducing effect.

The paper provides tools and methods for analyzing high-throughput single-molecule biophysics data using first-passage time and maximum likelihood estimation to model kinetic behavior.

This paper develops an in-situ force calibration method for magnetic tweezers and uses it to reveal how MTERF1 causes directional transcription termination through DNA unwinding and a kinetic barrier.

This study shows that the coronavirus helicase enhances RNA synthesis through duplex RNA by tenfold by forming a directional complex with the polymerase, revealing an active coupling mechanism that modulates replication dynamics.

This study shows that coronavirus polymerase template switching is driven by nsp13-helicase and is enhanced by remdesivir and molnupiravir, leading to defective RNA production.

The paper uses single-molecule magnetic tweezers to study how TAV2b-derived peptides affect dsRNA structure and stability, finding they underwind and stabilize the duplex.

This study shows MDA5 compacts dsRNA via ATP-dependent loop extrusion, stabilizing the structure through CARD domain oligomerization.