Craig E. Cameron

This paper shows SARS-CoV-2 can tolerate reduced proofreading activity but requires a minimal ExoN activity threshold for viability, leading to a mutator phenotype and reduced replication efficiency.

This study characterizes the kinetic mechanism and structural dynamics of Chikungunya virus nsP2 RNA helicase during nucleotide hydrolysis using biochemical and computational methods.

This paper develops an in-situ force calibration method for magnetic tweezers and uses it to reveal how MTERF1 causes directional transcription termination through DNA unwinding and a kinetic barrier.

This study shows that the coronavirus helicase enhances RNA synthesis through duplex RNA by tenfold by forming a directional complex with the polymerase, revealing an active coupling mechanism that modulates replication dynamics.

This study investigates the RNA unwinding mechanism of orthoflavivirus NS3 helicases, finding that they require translocation beyond the displaced strand and are stimulated by NS5 RdRp.

This study identifies a fold switch in ONNV nsP4 that regulates its conformation, RNA binding, and polymerase activity through proteolytic cleavage and fold switching.

This paper develops a computational pipeline to identify conserved antiviral target sites in alphaviruses using AI-driven methods, validating potential drug targets through mutagenesis.

This study identifies a minimal functional precursor of CHIKV nsP4 required for RNA synthesis and shows cleavage by nsP2 protease is essential for replication.

This study shows that coronavirus polymerase template switching is driven by nsp13-helicase and is enhanced by remdesivir and molnupiravir, leading to defective RNA production.

This study shows that arabinose nucleotides inhibit SARS-CoV-2 and poliovirus polymerases by causing long pauses due to the 2'-endo conformation of the incorporated nucleotides.

The paper describes the enantioselective resolution of a chemical probe targeting CHIKV nsP2 helicase, showing potent antiviral activity for one enantiomer and low activity for the other.

This study identifies a first-in-class inhibitor of alphavirus nsP2 helicase activity, showing broad antiviral activity and in vivo efficacy against chikungunya virus.

This paper investigates how enteroviral RNA-dependent RNA polymerases perform forced-copy-choice RNA recombination, showing the role of a plus-one intermediate and preference for CMP over other nucleotides.

This paper identifies and optimizes oxaspiropiperidine inhibitors targeting nsP2hel, showing nanomolar antiviral activity against multiple alphaviruses.

This study identifies a poliovirus nonstructural protein 3CD with an LIR motif critical for non-lytic spread via autophagosome incorporation and trafficking.

This study uses a microfluidic device to characterize lytic and non-lytic virus spread between single cells, finding non-lytic spread is faster and more efficient.

This paper identifies and characterizes RA-0002034, a covalent inhibitor of CHIKV nsP2 protease, showing potent inhibition and selectivity against alphaviruses.

This study develops a stochastic mechanistic model to analyze viral replication dynamics from single-cell poliovirus infection experiments, identifying early replication steps as key drivers of variability in viral growth.

This study develops a cell-based assay to investigate recombination in EV-A71, finding that strain-type, RNA diversity, RdRp, and sequence homology influence recombination frequency and mechanism.

The paper investigates PV-induced organelles by analyzing spatiotemporal dynamics of PI4P and membrane structures, suggesting distinct organelles for genome replication and virus assembly.

This study shows that poliovirus recombination is important for pathogenesis in animals but detrimental to replication in tissue culture, suggesting recombination aids viral spread within hosts.