Misha Klein

The paper provides tools and methods for analyzing high-throughput single-molecule biophysics data using first-passage time and maximum likelihood estimation to model kinetic behavior.

This study shows that coronavirus polymerase template switching is driven by nsp13-helicase and is enhanced by remdesivir and molnupiravir, leading to defective RNA production.

The paper shows that the SARS-CoV-2 polymerase core RTC undergoes a conformational change after RNA binding to become elongation-competent, which is essential for viral RNA synthesis.

The paper uses single-molecule magnetic tweezers to study how TAV2b-derived peptides affect dsRNA structure and stability, finding they underwind and stabilize the duplex.

This study shows MDA5 compacts dsRNA via ATP-dependent loop extrusion, stabilizing the structure through CARD domain oligomerization.

The paper constructs a kinetic model to predict CRISPR-Cas off-targeting based on mismatch positions and distances, explaining efficiency and specificity decoupling.