Quinte Smitskamp

The nucleoside analog CMX521 inhibits coronavirus RNA-dependent RNA polymerase via a two-pronged mechanism

This study identifies a two-pronged mechanism by which CMX521 inhibits SARS-CoV-2 RNA-dependent RNA polymerase through biochemical assays and single-molecule techniques.

A multimodal, correlative magnetic tweezers-TIRF platform for high-throughput single-molecule interrogations

This paper introduces a multimodal platform combining magnetic tweezers and TIRF for high-throughput single-molecule analysis, enabling real-time, parallel measurements of force, torque, fluorescence, and temperature.

Accurate single-bead force calibration in high-throughput magnetic tweezers reveals the mechanism of directional transcription termination by MTERF1

This paper develops an in-situ force calibration method for magnetic tweezers and uses it to reveal how MTERF1 causes directional transcription termination through DNA unwinding and a kinetic barrier.

Template switching by coronavirus polymerase requires helicase activity and is stimulated by remdesivir and molnupiravir

This study shows that coronavirus polymerase template switching is driven by nsp13-helicase and is enhanced by remdesivir and molnupiravir, leading to defective RNA production.

Single-molecule magnetic tweezers reveals that TAV2b-derived peptides underwind and stabilize double-stranded RNA

The paper uses single-molecule magnetic tweezers to study how TAV2b-derived peptides affect dsRNA structure and stability, finding they underwind and stabilize the duplex.

MDA5 generates compact ribonucleoprotein complexes via ATP-dependent single-stranded RNA loop extrusion

This study shows MDA5 compacts dsRNA via ATP-dependent loop extrusion, stabilizing the structure through CARD domain oligomerization.