Chenghua Gu

Spatial distribution of blood-brain barrier membrane proteins is controlled by sorting motifs and physiological signals in vivo

STED imaging quantifies luminal versus abluminal localization of blood-brain barrier proteins in vivo; tyrosine motifs drive abluminal sorting of OAT3 and MHC I, and CD31 shifts day to night.

HaloTrace: A spatiotemporally precise fluorescent readout of blood-brain barrier permeability in mice

HaloTrace provides a spatiotemporal readout of BBB permeability in mice by using a fluorescent ligand that specifically accumulates in the brain at sites of BBB dysfunction.

Canonical Wnt Signaling Suppresses Brain Endothelial Cell Transcytosis to Maintain Blood-Brain Barrier Integrity

This study shows that canonical Wnt signaling suppresses transcytosis in brain endothelial cells, maintaining blood-brain barrier integrity by regulating tight junctions and vesicle dynamics.

The secreted neuronal signal Spock1 regulates the blood-brain barrier

The paper identifies Spock1 as a neuronal signal that regulates blood-brain barrier permeability by altering extracellular matrix and vascular gene expression in zebrafish and mice.

Vascular and perivascular cell profiling reveals the molecular and cellular bases of blood-brain barrier heterogeneity

The paper profiles vascular and perivascular cells in different brain regions to reveal molecular and cellular bases of blood-brain barrier heterogeneity.

Cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target- and brain-region specific manner

The paper identifies that cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target- and brain-region specific manner, revealing heterogeneity in their function and connectivity.

Suppression of transcytosis regulates zebrafish blood-brain barrier development

The paper studies zebrafish blood-brain barrier development, finding that suppression of transcytosis is critical for BBB formation and that Mfsd2a plays a conserved role in this process.

Target-specific co-transmission of acetylcholine and GABA from a subset of cortical VIP+ interneurons

The paper identifies VIP/ChAT interneurons as a local source of cortical ACh and shows they co-transmit ACh and GABA to specific post-synaptic targets.