Christopher J. Kuffner

ProNotch converts extracellular protease activity into programmable transcriptional outputs by using a modular receptor system that responds to protease cleavage.

This paper introduces RNA-stabilized coat proteins for low-background live-cell RNA imaging, enabling sensitive visualization of single mRNAs and dual-color imaging of distinct RNA species.

This paper introduces a fluorescein-based SynNotch system to regulate gene expression in response to extracellular cues, demonstrating its use in controlling myogenic conversion.