John T. Ngo

ProNotch converts extracellular protease activity into programmable transcriptional outputs by using a modular receptor system that responds to protease cleavage.

This paper introduces iDARs, inducible deaminases that enable stimulus-responsive RNA editing, allowing controlled protein translation or transcript degradation in response to various triggers.

This paper introduces RNA-stabilized coat proteins for low-background live-cell RNA imaging, enabling sensitive visualization of single mRNAs and dual-color imaging of distinct RNA species.

This paper introduces a superresolution microscopy technique called centroid reassignment microscopy (CRM) that enables fast, robust, and protocol-compatible imaging of live samples.

The paper introduces protease-containing nanobodies controlled by antiviral drugs to manipulate intracellular protein-protein interactions.

This paper introduces a fluorescein-based SynNotch system to regulate gene expression in response to extracellular cues, demonstrating its use in controlling myogenic conversion.

The paper develops a system using viral proteases and antiviral drugs to control protein activity and gene modulation through allosteric regulation of Cre recombinase.

The paper develops drug-gated CAR circuits using NS3 protease domains and FDA-approved inhibitors to enhance CAR T cell therapy safety and specificity.

The paper introduces HaXS8-inducible systems for controlling synthetic biological functions in mammalian cells using SNAP-tag and HaloTag domains.

This paper introduces tension-tuned SynNotch receptors that convert mechanical forces into gene expression changes, enabling synthetic mechanosensing and intercellular force detection.