Weikang Wang

Sloppiness and Action Constraint in Cell State Transitions: Are Single Cells Sloppy?

The paper analyzes single-cell dynamics during state transitions, finding that cell states are sensitive to few 'stiff' parameters and robust to many 'sloppy' parameters, suggesting a principle of least action.

LivecellX: Corrective Deep Learning for Object-Oriented Single-Cell Analysis in Live-Cell Imaging

LivecellX corrects segmentation and tracking errors in live-cell imaging using deep learning and provides tools for lineage reconstruction and dynamic analysis.

Transiently increased intercommunity regulation characterizes concerted cell phenotypic transition

The paper analyzes single-cell RNA sequencing data using transition path theory to study gene regulation during cell phenotypic transitions, finding increased intercommunity regulation and GRN frustration.

Live cell imaging and analysis reveal cell phenotypic transition dynamics inherently missing in snapshot data

The paper develops a live-cell imaging platform to study phenotypic transitions, revealing dynamics missed by snapshot data.

Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-in

This paper develops a modular Gibson assembly method for efficient and cost-effective CRISPR donor DNA construct generation, showing improved assembly efficiency compared to conventional methods.

Pathway crosstalk enables cells to interpret TGF-β duration

The paper shows that TGF-β signaling duration is interpreted through pathway crosstalk that controls SNAIL1 expression.