Hidenori Ichijo

The paper develops methods to improve the solubility and stability of intrabodies by optimizing linker design, domain orientation, and charge properties, enabling effective intracellular targeting of cytoplasmic proteins.

This study shows that proteasome activity inactivates ASK3 by degrading ASK1, linking proteasome function to cell survival under hyperosmotic stress.

The paper identifies the ASK family as a key signaling molecule linking PKA to MAPK in mature brown adipocytes and shows phosphorylation events critical for their activation.

The paper presents the cryo-EM structure of the human LRRC8A protein, revealing its trimer-of-dimers assembly and structural features relevant to its function as a volume-regulated anion channel.