Daryl A. Bosco

Breaking β-sheets in FUS prion-like domain preserves phase separation and function but prevents aggregation and toxicity

This study introduces β-sheet breaking proline residues into FUS prion-like domain to prevent aggregation and toxicity while preserving phase separation and function.

Reliable repurposing of the antibody interactome inside the cell

The paper develops methods to improve the solubility and stability of intrabodies by optimizing linker design, domain orientation, and charge properties, enabling effective intracellular targeting of cytoplasmic proteins.

TDP-43 dysfunction leads to impaired proteostasis and predisposes mice to worse neurological outcomes after brain injury

This study shows that TDP-43 dysfunction impairs proteostasis and worsens neurological outcomes after brain injury in mice.

Expression of ALS-PFN1 impairs vesicular degradation in iPSC-derived microglia

This study shows that ALS-linked PFN1 mutations impair vesicular degradation in iPSC-derived microglia by disrupting autophagy, which can be mitigated by Rapamycin.

A reference induced pluripotent stem cell line for large-scale collaborative studies

The paper identifies and characterizes a reference iPSC line, KOLF2.1J, for standardized use in collaborative studies across multiple differentiation protocols and assays.

FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for Gria2 mRNA processing

The paper shows that FUS/TLS translocates from the nucleus to the cytoplasm during excitotoxic stress via a calcium-dependent mechanism and is required for Gria2 mRNA processing.