Takeshi Yokoyama

Selective Recognition, Covalent Linkage and Chemical Exchange of Artificial Cells through Engineered α-Hemolysin

This paper engineers α-hemolysin to enable selective recognition, covalent linkage, and chemical exchange between artificial cells through a single protein system.

In-Cell Protein Crystallization via a Locally Flexible 24-mer Assembly Precursor

This study defines the assembly pathway of CipB, showing that a 24-mer precursor forms and packs into a cubic lattice, with N-terminal flexibility enabling crystallization.

Modulation of translational elongation by montanine alkaloids preferentially inhibits RNA viruses

This study identifies montanine as a potent antiviral compound that inhibits RNA viruses by targeting translational elongation and GCN1-related stress responses.

Structural basis for bispecific antibody design: arrangement of domain linkage produces activity enhancement

This study determines the cryo-EM structures of two bispecific antibody types and shows that domain linkage rearrangement leads to activity enhancement by altering complex shape and dynamics.

The glomerulus as a selective gate for tumor-derived extracellular vesicles in urine

This study shows the glomerulus actively transports tumor-derived extracellular vesicles into urine, revealing a mechanism for their excretion and potential use in cancer diagnostics.

Structural insight into bacterial co-transcriptional translation initiation

The paper reports cryo-EM structures of the translation initiation complex assembled on transcribing RNA polymerase, showing how mRNA is protected during co-transcriptional translation initiation.

Structural Basis for eIF2B Inhibition in Integrated Stress Response

The paper determines cryo-EM and crystal structures of eIF2B with phosphorylated and unphosphorylated eIF2 to reveal how eIF2 phosphorylation inhibits eIF2B activity.

Cryo-EM structure of the volume-regulated anion channel LRRC8

The paper presents the cryo-EM structure of the human LRRC8A protein, revealing its trimer-of-dimers assembly and structural features relevant to its function as a volume-regulated anion channel.