Stephan Gruber

This study shows the segment-capture model of SMC complexes can explain obstacle bypassing and loop extrusion, and proposes experiments to test the model.

This study shows that GcrA, a methylation-dependent regulator in Agrobacterium tumefaciens, controls replication timing and cell division by coordinating RepABC origins and divisome gene expression.

This study uses synthetic nanobodies to identify functional regions of the Bacillus subtilis SMC complex, revealing the mid-region of the coiled coil as critical for DNA folding.

The paper shows that SMC hinge domains selectively allow ssDNA to bypass obstacles during DNA loop extrusion, revealing a conserved mechanism across SMC complexes.

This study shows that TelN protects hairpin telomeres from Mre11-Rad50 degradation in E. coli by sequence-specific DNA binding and protein-protein interactions, independent of its resolution activity.

The paper presents structures of a Lamassu defence system from Salmonella enterica, revealing how LmuA nuclease effector is activated by oligomerization and DNA cleavage.

This study shows BisD, a ParB-like protein, coordinates gene activation and DNA segregation in ICEclc transfer competence development in Pseudomonas putida.

This study determines the structure of a type II Wadjet SMC complex from Neobacillus vireti, revealing structural differences from type I systems and insights into prokaryotic SMC evolution.

The paper measures the twist induced by eukaryotic SMC proteins during DNA loop extrusion and finds all three SMC complexes induce a consistent -0.6 twist per step.

This paper introduces a cloning method for rapidly generating expression constructs of multi-subunit protein complexes in various hosts.

This study shows DNA supercoiling enhances ParB-DNA condensation, forming partition complexes at lower ParB concentrations than on relaxed DNA.

The paper reveals how SMC JET nuclease recognizes and cleaves plasmid DNA by mechanically bending residual unextruded DNA, identifying key structural elements involved in the process.

The paper shows that all eukaryotic SMC complexes extrude DNA asymmetrically, with direction switches observed in cohesin and SMC5/6, linked to NIPBL turnover and stabilized by STAG1.

The paper shows JetABCD SMC complexes restrict small circular plasmids by cleaving them, while linear plasmids are not restricted, and presents a structural model for this process.