Kotaro Sasaki

This study generates spermatogonia from human and non-human primate pluripotent stem cells using xenogeneic testes, providing models for male infertility research.

The paper integrates single-cell and spatial multiomic data to show widespread reactivation of developmental programs in diseased human hearts, with insights into transcriptional factors and localized fetal reactivation.

The paper maps human kidney development using single-cell RNA sequencing and spatial transcriptomics, revealing fate decisions influenced by the local microenvironment.

This paper develops a human pluripotent stem cell-derived adrenal organoid system that recapitulates adrenal zonation and demonstrates its utility for studying adrenal development and disease.

This study shows that an ENL mutation disrupts kidney development by altering gene regulation, leading to developmental defects and early death in mice, and suggests potential therapeutic strategies.

This study maps seminoma transcriptomes and epigenetic features to the normal human germline trajectory, revealing similarities to primordial germ cells and identifying gene dosage effects in a patient-derived model.

This study uses CRISPR screening to identify genes critical for human primordial germ cell-like cell development, finding TCL1A regulates AKT-mTOR signaling and protein synthesis.

This study identifies a mechanical pacemaker in the kidney stroma that synchronizes nephron formation with ureteric bud branching.

This study establishes methods to generate marmoset primordial germ cell-like cells from iPSCs, revealing a primate-specific germline transcriptional network and potential for in vitro gametogenesis modeling.

This study generates human fetal zone adrenal cortex-like cells from induced pluripotent stem cells, revealing key signaling pathways and transcription factors involved in adrenocortical specification and steroidogenesis.

The paper identifies the adrenal cortex's origin in adrenogenic coelomic epithelium, distinct from gonadogenesis, in humans and cynomolgus monkeys.

This study identifies LTR5_Hs, a hominoid-specific ERV, as rewiring the gene regulatory network shared between primordial germ cells and naive pluripotent cells through enhancer activity.

This study establishes an in vitro protocol to generate human prospermatogonia-like cells from induced pluripotent stem cells and characterizes their lineage trajectory using single-cell RNA-sequencing.