Karl J. Lewis

This study compares two-photon and three-photon microscopy in ex vivo mouse bones, finding 3P provides better image quality beyond 50 micrometers and enables visualization of marrow cells through intact bone.

This study shows that cholesterol depletion in osteocytes reduces membrane dynamics and alters mechanosensitivity, with sex-dependent effects on Ca2+ signaling in response to mechanical loading.

This paper develops a real-time axial motion compensation system for intravital two-photon imaging of mechanically loaded bone, improving image quality and data reliability.

The paper studies endocytosis in osteocytes in vivo, finding sex-specific differences in dynamin-dependent endocytosis and nanoparticle uptake patterns.

This paper introduces a modified BABB clearing protocol to improve imaging of calcified tissues by addressing their opacity and rigidity.

This study identifies a novel mechanosensory complex in osteocytes involving perlecan and α2δ1 subunit, showing that gabapentin disrupts their interaction and impairs bone mechanosensation.