Chris B. Schaffer

Depth-Dependent Advantages of Three-Photon Microscopy for Imaging Through Intact Murine Cortical Bone and Into the Marrow

This study compares two-photon and three-photon microscopy in ex vivo mouse bones, finding 3P provides better image quality beyond 50 micrometers and enables visualization of marrow cells through intact bone.

Reprogrammed neutrophils with impaired transit mechanics drive multi-organ capillary stalling after stroke

This study identifies reprogrammed neutrophils with impaired transit mechanics as a driver of multi-organ capillary stalling after stroke, showing therapeutic potential through Fgr inhibition.

Scattering-enabled epi-quantitative phase imaging reveals subcellular detail in organoids and deep mouse brains

This paper introduces SEEQPI, a label-free imaging technique that enables subcellular resolution and deep imaging in turbid tissues, demonstrating its application in organoids and mouse brains.

Label-free Tracking of Subcortical White Matter Degradation In Vivo Using Third Harmonic Generation Microscopy in a Mouse Model of Multiple Sclerosis

This study uses THG microscopy to track subcortical myelin degradation in a mouse MS model, finding increased intranodal distances and a new quantitative metric for early detection.

Psilocybin Prolongs the Neurovascular Coupling Response in Mouse Visual Cortex

This study shows psilocybin prolongs neurovascular coupling in mouse visual cortex, affecting blood flow without altering neural responses, and suggests implications for interpreting human neuroimaging data.

Neutrophil arrest in myocardial capillaries drives hypoxia and impairs diastolic function in a mouse model of heart failure with preserved ejection fraction

This study identifies neutrophil arrest in myocardial capillaries as a driver of hypoxia and impaired diastolic function in a mouse model of HFpEF, showing that reducing neutrophil arrest improves cardiac function.

Bidirectional Regulation of Motor Circuits Using Magnetogenetic Gene Therapy

This paper develops magnetogenetic tools to bidirectionally regulate specific neuronal circuits in vivo using magnetic fields and shows their efficacy in motor control and Parkinson's models.

Circumscribing laser cuts attenuate seizure propagation in a mouse model of focal epilepsy

In a mouse model of focal epilepsy, sub-surface laser-produced cuts encircling the seizure focus attenuate propagation without behavioral impairment.

Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cord in vivo

Vascular oxidative stress causes neutrophil arrest in brain capillaries, leading to decreased cerebral blood flow and contributing to memory impairment in a mouse model of Alzheimer's disease

This study shows that inhibiting NOX2 reduces capillary stalling and inflammation in Alzheimer's mice, improving memory and blood flow.

Hypoxia induced carbonic anhydrase mediated dorsal horn sensory neuron activation and induction ofneuropathic pain

The paper shows that hypoxia in the spinal dorsal horn, caused by vascular issues, activates sensory neurons and leads to neuropathic pain, which can be reversed by inhibiting carbonic anhydrase.

Inhibition of peripheral VEGF signaling rapidly reduces leucocyte obstructions in brain capillaries and increases cortical blood flow in an Alzheimers disease mouse model

This study shows that inhibiting VEGF signaling in AD mice reduces leucocyte adhesion in brain capillaries, increases blood flow, and improves BBB integrity.

Voluntary running does not increase capillary blood flow but promotes neurogenesis and short-term memory in the APP/PS1 mouse model of Alzheimers disease

This study shows that voluntary running improves memory and neurogenesis in Alzheimer's mouse models but does not increase cerebral blood flow or reduce amyloid-beta deposits.

High fat diet worsens pathology and impairment in an Alzheimers mouse model, but not by synergistically decreasing cerebral blood flow

The paper shows that a high fat diet worsens Alzheimer's disease symptoms in mice but does not synergistically reduce cerebral blood flow, suggesting capillary stalling is not the mechanism linking diet and disease severity.

Neutrophil-mediated dynamic capillary stalls in ischemic penumbra: persistent traffic jams after reperfusion contribute to injury

The paper identifies dynamic capillary flow stalls in mice after stroke reperfusion, mediated by neutrophils, which impair circulation and contribute to penumbral injury.

Increasing cerebral blood flow improves cognition into late stages in Alzheimer’s disease mice

A single injection of anti-Ly6G improves short-term memory in younger Alzheimer's mice but not older ones, while still increasing cerebral blood flow in older mice.

Neutrophil adhesion in brain capillaries contributes to cortical blood flow decreases and impaired memory function in a mouse model of Alzheimer’s disease

This study identifies neutrophil adhesion in brain capillaries as a cause of reduced cerebral blood flow and impaired memory in an Alzheimer's mouse model, showing that targeting this adhesion improves both CBF and memory.

Aspirin treatment does not increase microhemorrhage size in young or aged mice

This study investigates whether aspirin affects microhemorrhage size in young and aged mice, finding no significant difference compared to controls.