MuSpAn, a Python library of over 100 spatial methods, quantifies multiscale tissue organisation and maps cancer-associated fibroblast subtypes to distinct immune niches in mouse colorectal tumours.

Nature Communications · Aug 13, 2026Peer-reviewedCell Biology✦ In-depth digest

Spatial remodeling of the thymic stroma with age disrupts niches for T cell selection and tolerance

Andreas Tarcevski, Fatima Dhalla, Joshua W. Moore, Saulius Žuklys, Anja Kusch, Liliana Tchernev, Joshua Grier, Stefano Maio, Amir Khan, Thomas Barthlott, Mary E. Deadman, Adam E. Handel, Helen Mary Byrne, Georg A. Holländer

This study maps spatial changes in the aging mouse thymus, revealing stromal reorganization that disrupts T cell selection and tolerance.

bioRxiv · Aug 5, 2026 · PreprintImmunology

netPCF: Geometry-Aware Pair Correlation Functions for Spatial Biology

Joshua W. Moore, Joshua A. Bull, Helen Mary Byrne

The paper introduces netPCF, a geometry-aware method for quantifying spatial organisation on complex biological domains, showing its utility in biological applications.

bioRxiv · Jul 7, 2026 · PreprintBioinformatics

Stromal Prostaglandin is a Dominant Spatial Regulator of Cell-fate Plasticity in Colorectal Cancer

Corinne Molyneux, Rhianna O’Sullivan, Eoghan J. Mulholland-Illingworth, Joshua W. Moore, Nick Li, Petra Vlckova, Raheleh Amirkhah, Aurélie Dobric, Shauna Crampsie, Alistair Wilkinson, Jack Langley, Marta Labarquilla Alonso, Ashley Campbell, Jeroen Claus, Smita Krishnaswamy, , Christopher J. Tape

This study identifies stromal prostaglandin E2 as a key regulator of cell-fate plasticity in colorectal cancer, showing it converts chemosensitive stem cells into chemorefractory, prometastatic cells.

bioRxiv · Jun 18, 2026 · PreprintCancer Biology

Integrating diverse statistical methods to analyse stage-discriminatory cell interactions in colorectal neoplasia

Joshua A. Bull, Eoghan J. Mulholland, Joshua W. Moore, Jesús J. Bosque, Bernadette J. Stolz, Joseph Boen, Holly R. Eggington, Hayley L. Belnoue-Davis, Helen J S Jones, Chandler Gatenbee, Alexander R.A. Anderson, Alistair Easton, Peter Martin Todd, Chris Cunningham, Stephen Taylor, , Simon John Leedham

The paper develops spatial descriptors using mathematical tools to analyze cell interactions in colorectal cancer progression and demonstrates their utility in identifying disease-related pathways.

bioRxiv · Jun 2, 2024 · PreprintCancer Biology