Andreas Kaczmarczyk

This paper introduces a self-regulating CRISPRi system that reduces leakiness while maintaining inducible gene repression, enabling portable and multiplexed applications.

The paper shows that opposing range-dependent interactions between microbial exoproducts create complex spatial patterns of antibiotic tolerance in biofilms.

The paper develops genetically-encoded biosensors to monitor antibiotic concentrations inside bacterial cells and tissues in real-time, revealing antibiotic exposure dynamics and penetration in infection models.

This study identifies pathways in P. aeruginosa that confer resistance to T6SS effectors from Acinetobacter baylyi and Vibrio cholerae, revealing links between T6SS resistance and antibiotic susceptibility.

The paper uses CRISPRi screens to identify metabolic vulnerabilities in P. aeruginosa PAO1 under different conditions, revealing key genes for targeting chronic infections.