Author

Joshua J. Brewer

Rockefeller UniversityORCID 0000-0002-6737-7828Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:chromatin & epigenetic regulation· 1 Assay:cryo-EM· 1 Organism:E. coli· 1 Research Focus:protein folding & dynamics· 1 Assay:structural biology· 1

Frequent Co-Authors

Elizabeth A. CampbelliD· 3 shared Seth Andrew DarstiD· 3 shared Rachel Anne MooneyiD· 2 shared Robert C. LandickiD· 2 shared Barbara BoschiD· 1 shared Brian T. ChaitiD· 1 shared Eliza LlewellyniD· 1 shared Expery O. OmolloiD· 1 shared

Activity by Year

2022-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 30 mutual co-author links

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3 shared papersBiophysics

Elizabeth A. CampbellORCID

Mutual co-authors in cluster

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3 papers

Recent·Most cited

Structural basis for loading of Transcription Repair-Coupling factor Mfd onto stalled elongation complexes

Joshua J. Brewer, Eliza Llewellyn, James Chen, E. Campbell, Seth Andrew Darst

This study resolves the structural basis of Mfd loading onto stalled elongation complexes by visualizing intermediates at 3.5 Å resolution using cryo-electron microscopy.

Nucleic Acids Research · Jul 3, 2026Peer-reviewedBiophysics

RapA opens the RNA polymerase clamp to disrupt post-termination complexes and prevent cytotoxic R-loop formation

Joshua J. Brewer, Koe Inlow, Rachel Anne Mooney, Barbara Bosch, Paul Dominic B. Olinares, Leandro Pimentel Marcelino, Brian T. Chait, Robert C. Landick, Jeff Gelles, Elizabeth A. Campbell, Seth Andrew Darst

The paper shows RapA disrupts post-termination complexes in E. coli to prevent R-loop formation and maintain genome stability.

Nature Structural & Molecular Biology · Jan 8, 2025Peer-reviewedBiophysics

Structural and functional basis of the universal transcription factor NusG pro-pausing activity in Mycobacterium tuberculosis

Madeleine Delbeau, Expery O. Omollo, Ruby Froom, Steven Koh, Rachel Anne Mooney, Mirjana Lilić, Joshua J. Brewer, Jeremy M. Rock, Seth Andrew Darst, Elizabeth A. Campbell, Robert C. Landick

This study identifies MtbNusG as a pro-pausing transcription factor that stabilizes paused RNAP through specific structural interactions, with implications for NusG function across domains of life.

bioRxiv · Oct 21, 2022 · PreprintMolecular Biology