James Chen

The paper uses AI-based protein-protein interaction predictions to characterize protein function in mycobacterial pathogens, identifying key interactions and functions for previously uncharacterized proteins.

This study resolves the structural basis of Mfd loading onto stalled elongation complexes by visualizing intermediates at 3.5 Å resolution using cryo-electron microscopy.

This study creates a single-cell atlas of pig-to-monkey kidney xenotransplantation, identifying macrophage chimerism and an IFN-ε-driven immune niche that protects the graft.

The paper identifies Rv2571c as a mycobacterial ion channel that mediates pyrazinamide resistance by regulating cytoplasmic acidification.

The paper performs a genome-wide CRISPRi screen in Mycobacterium smegmatis to uncover novel gene functions and drug targets related to the cell envelope.

The paper identifies CoaX, a pseudokinase, and pantothenate as regulators of proteasomal degradation of the pantothenate synthesis enzyme PanB in M. tuberculosis.

This study uses time-resolved cryo-EM to visualize early intermediates in bacterial RNA polymerase promoter melting, revealing key steps in the formation of the transcription-competent open complex.

The paper identifies NAT10's role in cancer through tRNA ac4C modification, showing RNPS1 stabilizes NAT10 and enhances translation efficiency via the AP-1 pathway.