Alexander P. Hynes

Growth phase-dependent priming of galactose metabolism in Escherichia coli

This study shows that prior growth phase in Escherichia coli influences its metabolic response to galactose, revealing a history-dependent behavior regulated by GalR and GalS.

Trimethoprim suppress phage activity and reduces toxin production in a clinical STEC strain, even in the presence of DNA-damaging antibiotics

High-throughput screening reveals diverse lambdoid prophage inducers

This study identifies novel prophage inducers through high-throughput screening, revealing that bleomycin is a potent and broad-spectrum inducer across multiple phages and bacterial hosts.

CRISPR adaptation in Streptococcus thermophilus can be driven by phage environmental DNA

This study shows that environmental DNA (eDNA) from phages drives CRISPR immunity in Streptococcus thermophilus by enhancing phage-immune colony generation, with effects dependent on phage specificity and environmental conditions.

Temperate phages increase antibiotic effectiveness in a Caenorhabditis elegans infection model

This study shows that temperate phages can enhance antibiotic effectiveness in vivo using C. elegans, demonstrating 'temperate phage antibiotic synergy' (tPAS) by reducing phage dormancy and improving infection outcomes.

Temperate phage-antibiotic synergy is widespread, but varies by phage, host, and antibiotic pairing

This study shows that temperate phages can synergize with antibiotics to reduce bacterial load in Pseudomonas aeruginosa, but this synergy depends on specific phage-antibiotic and phage-host pairings.

Micro-plaque assays: A high-throughput method to detect, isolate, and characterize bacteriophages

This paper introduces micro-plaque assays to improve phage isolation and characterization, showing they can isolate 21 unique phages from one sample and identify phage susceptibility in clinical strains.