Yang Sun

A genome-wide CRISPR activation map of surface protein expression in human CD4 T cells

This paper introduces SCITO-Perturb-seq, a CRISPRa platform that maps surface protein regulation in CD4 T cells, finding 16% of activated genes alter surface protein expression.

A20's Linear Ubiquitin Binding Motif Restrains Pathogenic Activation of TH17/22 cells and IL-22 Driven Enteritis

This study shows that A20's M1 ubiquitin binding motif suppresses TH17/22 cell activation and IL-22-driven enteritis by regulating RORγt and epigenetic changes in T cells.

Reference-free multiplexed single-cell sequencing identifies genetic modifiers of the human immune response

The paper introduces clue, a reference-free framework for multiplexed single-cell sequencing to identify genetic modifiers of immune responses using SNP-based clustering and eQTL mapping.

SCITO-seq: single-cell combinatorial indexed cytometry sequencing

SCITO-seq enables cost-effective profiling of over 10^5 cells using combinatorial indexing with dsc-seq, demonstrating feasibility in mixed cell lines and human lymphocytes.