Raghav Shroff

Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes

This study evolves a T7 RNA polymerase variant with a capping enzyme to enable efficient gene expression in eukaryotes, showing improved performance in yeast and mammalian cells.

A structure-based deep learning framework for protein engineering

This paper introduces a 3D convolutional neural network for protein engineering that identifies gain-of-function mutations, validated by experimental improvements in protein stability.