Shengying Li

This study reports cryo-EM structures of comammox AMO from Nitrospira inopinata and identifies its catalytic and inhibitor-responsive architecture, revealing a methyl-PQ-linked mechanism and stress response to nitrification inhibitors.

The paper structures and engineers deep-sea halophilic PET hydrolases to improve their salt tolerance and catalytic efficiency for industrial applications.

Archaeal ammonia monooxygenase (AMO) is a cup-shaped homotrimer using a mononuclear CuD site; inhibitors bind bacterial AMO's dicopper center directly but apparently inactivate archaeal AMO indirectly by relocating copper.

This paper engineers terminal deoxynucleotidyl transferase (TdT) to improve enzymatic DNA synthesis efficiency and scalability.

The paper introduces RACE, a method for rapidly evolving NRPSs through recombineering, generating novel peptides and uncovering new recombination sites.

The paper develops ACCESS, a multimodal graph neural network to annotate functional dark matter proteins from extreme environments beyond homology-based methods.

This paper identifies the enzymatic mechanism for forming the gamma-thiolactone ring in thiolactomycin biosynthesis, revealing the roles of TlnCCy and TlnA.

The paper analyzes gene family evolution across nearly all known Saccharomycotina yeast species, finding distinct evolutionary patterns with gene loss linked to metabolic and genomic changes.

This study reveals that CYP152 peroxygenases can degrade aromatic pollutants without a carboxyl group, showing new substrate recognition and catalytic mechanisms.

This paper introduces FISH-scRACS-Seq, a method to identify and sequence single cells with specific metabolic functions, revealing a new cyclohexane-degrading enzyme system in marine bacteria.

The paper identifies residue E299 as critical for the divergent catalytic functions of DnrK and RdmB in anthracycline biosynthesis, revealing distinct mechanisms for 10-hydroxylation and 4-O-methylation.