Wim Pomp

A Cooperative Mechanism of Eukaryotic Transcription Factor Target Search

This study directly visualizes eukaryotic transcription factor target search in living cells and finds that efficient localization occurs near the diffusion limit without requiring facilitated diffusion, instead relying on cooperative self-interactions mediated by intrinsically disordered regions.

Transcription factor exchange enables prolonged transcriptional bursts

The paper shows that transcription factor exchange prolongs transcriptional bursts in yeast by increasing burst duration through frequent TF exchange at multiple binding sites.

DNA supercoiling restricts the transcriptional bursting of neighboring eukaryotic genes

The paper shows DNA supercoiling inhibits transcriptional bursting of neighboring genes in yeast, differing from bacterial mechanisms.

Transcription factor clusters enable target search but do not contribute to target gene activation

This study investigates how TF clustering affects gene expression by analyzing Gal4 in budding yeast, finding that clustering aids target search but does not contribute to activation.