Tineke Laura Lenstra

This study directly visualizes eukaryotic transcription factor target search in living cells and finds that efficient localization occurs near the diffusion limit without requiring facilitated diffusion, instead relying on cooperative self-interactions mediated by intrinsically disordered regions.

The paper investigates how Fpt1 is recruited to tRNA genes in budding yeast, revealing a dual role of TFIIIB and TFIIIC in its binding under different RNAPIII conditions.

The paper shows that transcription factor exchange prolongs transcriptional bursts in yeast by increasing burst duration through frequent TF exchange at multiple binding sites.

The paper identifies Fpt1 as a chromatin-associated regulator that promotes RNA Polymerase III eviction from tRNA genes in response to nutrient changes in yeast.

The paper uses dynamic epistasis analysis to show how chromatin remodeling factors and transcription factor binding regulate transcriptional bursting in budding yeast.

The paper shows DNA supercoiling inhibits transcriptional bursting of neighboring genes in yeast, differing from bacterial mechanisms.

This study investigates how TF clustering affects gene expression by analyzing Gal4 in budding yeast, finding that clustering aids target search but does not contribute to activation.

The paper shows that transcriptional regulation in yeast is cyclic and irreversible, violating thermodynamic equilibrium.

The paper shows that transcriptional control and chromatin architecture at the Sox2 locus are decoupled, with distinct regulatory mechanisms.

The paper identifies Hda1C as a repressor of divergent non-coding RNA transcription in budding yeast by reducing transcription initiation frequency and histone acetylation.

The paper investigates how CTD length and disorder affect transcriptional bursting using smFISH, live imaging, and RNA-seq, finding that length modulates burst size and frequency while disorder mediates CTD-CTD interactions essential for transcription.

This study uses single-molecule imaging to show that Gal4 dwell time determines transcriptional burst size in yeast, with nucleosomes reducing dwell time and affecting transcriptional bursting kinetics.