Joseph V.W. Meeussen

A Cooperative Mechanism of Eukaryotic Transcription Factor Target Search

This study directly visualizes eukaryotic transcription factor target search in living cells and finds that efficient localization occurs near the diffusion limit without requiring facilitated diffusion, instead relying on cooperative self-interactions mediated by intrinsically disordered regions.

Recruitment of Fpt1 to tRNA genes requires TFIIIB and the N-terminal TPR array of TFIIIC subunit {tau}131

The paper investigates how Fpt1 is recruited to tRNA genes in budding yeast, revealing a dual role of TFIIIB and TFIIIC in its binding under different RNAPIII conditions.

Transcription factor exchange enables prolonged transcriptional bursts

The paper shows that transcription factor exchange prolongs transcriptional bursts in yeast by increasing burst duration through frequent TF exchange at multiple binding sites.

Transcription factor clusters enable target search but do not contribute to target gene activation

This study investigates how TF clustering affects gene expression by analyzing Gal4 in budding yeast, finding that clustering aids target search but does not contribute to activation.