Author
San Francisco General HospitalORCID 0000-0002-7893-8416Unclaimed ORCID profile · follow to get notified of new preprints
5 papers
Recent·Most cited
Weihao Zheng, Jason D. Limberis, Zachary P. Howard…·Joel D. Ernst
The paper identifies impaired IFNγ responsiveness in CD11clo monocyte-derived lung cells as a mechanism allowing Mycobacterium tuberculosis persistence during chronic infection.
Jason D. Limberis, John Z. Metcalfe
The paper reviews bead beating methods for Mycobacterium tuberculosis lysis, identifies protocol variability, and highlights the need for standardization to improve reproducibility.
Jason D. Limberis, John Z. Metcalfe
URAdime identifies problematic primers in sequencing data by detecting dimers and super-amplicons post hoc.
Jason D. Limberis, Roland J Nagel, Soumitesh Chakravorty…·John Z. Metcalfe
spotPCR improves mutation detection by streamlining UMI tagging during PCR, enhancing sensitivity and specificity without requiring multiple labor-intensive steps.
Jason D. Limberis, Alina Nalyvayko, J. Ernst, John Z. Metcalfe
The paper presents streamlined methods for generating circular DNA from the rv0678 gene to improve genotypic bedaquiline resistance testing in Mycobacterium tuberculosis.